Coding
YebF-CenA

Part:BBa_K2922001:Design

Designed by: Ruofan Yang   Group: iGEM19_XMU-China   (2019-10-13)


Endoglucanase A fused at N-terminal with YebF secretion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 412
    Illegal NotI site found at 1556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1469
    Illegal BamHI site found at 605
    Illegal XhoI site found at 967
    Illegal XhoI site found at 1216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 747
    Illegal NgoMIV site found at 1672
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651


Design Notes

Overlap extension PCR from yebf gene and endoglucanase A gene.


Source

YebF comes from Escherichia coli (Strain K12) and cenA is from Cellulomonas fimi.

References